Composite

Part:BBa_K227009:Design

Designed by: WashU iGEM 2009   Group: iGEM09_Wash_U   (2009-10-17)

pucpro+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 26
    Illegal XhoI site found at 185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 574
    Illegal BsaI site found at 583
    Illegal BsaI.rc site found at 1301


Design Notes

puc promotor is not active in E.coli puc promotor is constitutively active in Rhodobacter sphaeroides at low oxygen tension.

Source

registry parts

References